Serine proteases of the complement system.

Detection of the Keratinolytic Activity of Agriculture and Mount Barker Strain Dermatophilus congolensis Serine Proteases.

P 144: Sunflower Mannose binding Lectin-Associated Serine Protease Inhibitor-1 (SFMI-1) and -2: Significant Inhibitors of Mannose binding Lectin Pathway which Helps in Multiple Sclerosis Treatment

One of the important parts of innate immunity is complement system that occurs in three different ways; the classic, the alternative and the lectin pathway. The four pattern recognition molecules that have been identified till now are Mannose binding lectin (MBL), a component of lectin pathway, and three ficolins (ficolin1,-2 and -3) which compound to the carbohydrates of the cell surface. MBL ...

Quantitative characterization of the activation steps of mannan-binding lectin (MBL)- associated serine proteases (MASPs) points to the central role of MASP-1 in the initiation of complement lectin pathway

Background: Autoactivation of initiator proteases of complement is a two-step process. Results: Autoactivation, and possible crossactivation steps of complement lectin pathway proteases were quantified. Conclusion: Only MASP-1 can autoactivate rapidly, and MASP-2 is activated by MASP-1. Significance: The determined kinetic data are helpful to interpret activation scenarios for the lectin pathwa...

Staphylococcal proteases aid in evasion of the human complement system.

Staphylococcus aureus is an opportunistic pathogen that presents severe health care concerns due to the prevalence of multiple antibiotic-resistant strains. New treatment strategies are urgently needed, which requires an understanding of disease causation mechanisms. Complement is one of the first lines of defense against bacterial pathogens, and S. aureus expresses several specific complement ...

Isolation and Characterization of Thermophilic Alkaline Proteases Resistant to Sodium Dodecyl Sulfate and Ethylene Diamine Tetraacetic Acid from Bacillus sp. GUS1

Thermophilic Bacillus sp. GUS1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis. The enzymes were stable in the alkaline pH range (8.0-12.0), with the optimum temperature and pH range of the proteases being 70ºC and 6.0-12.0, respectively. All th...